Erd ÖS, T., and A. Ullmann: Effect of streptomycin on the incorporation of amino-acids labelled with carbon-14 into ribonucleic acid and protein in a cell-free system of Mycobacterium.
Sequence clusters. Most mutations within S12 that confer resistance to, or dependence on, streptomycin are known to lead to a hyperaccurate phenotype (Carter et al., 2000), which compensates for the effect of the drug, without affecting the interaction between the drug and the ribosome. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well.
Streptomycin acts on the ribosome, inhibiting the translation of mRNA and thereby disrupting protein synthesis. Rarer ones are neuromuscular blockade and hypersensitivity reactions … Mutations including those in the 30S subunit arise readily in response to isolated streptomycin therapy and lead to drug resistance. You are using a version of browser that may not display all the features of this website. Proteomes. Required for streptomycin resistance (PubMed:3357770). Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))
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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).
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This section provides general information on the entry.
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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier.
DNA Data Bank of Japan; a nucleotide sequence database, Database of comparative protein structure models, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Die aerob lebenden Streptomyceten gehören zu der großen Gruppe der Aktinobakterien. The information is filed in different subsections.
The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64)
Relationships between antibiotic and protein kinases. Science 12 Apr 1968: 198-199 . with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. Annotation systems. UniRef. The 16S rRNA region mutated perturbs a pseudoknot structure in a region which has been linked to ribosomal S12 protein. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. Gene ID: 13982014, updated on 16-Dec-2016.
Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
AU - Ludvigsen, Jane. A streptomycin resistant mutant grew equally on both the TBAB and TBAB+Strep plates, and intermediate mutants grew better on the Strep plate than the plate without Streptomycin. 118 route de Narbonne, 31062 Toulouse Cedex, France. Help. For active tuberculosis it is often given together with isoniazid, rifampicin, and pyrazinamide. It was previously known that streptomycin resistance came from mutations in the rpsL (ribosomal protein L) gene, as streptomycin's mode of action is to bind to this protein and restrict ribosome function. N2 - The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. Streptomycin was discovered in 1943 from Streptomyces griseus. The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. The version number for both the entry and the canonical sequence are also displayed.
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This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (reviewed) or to the computer-annotated TrEMBL section (unreviewed).
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UniProtKB Keywords constitute a controlled vocabulary with a hierarchical structure. A total of 128 clinical isolates were tested by the phage assay for isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance while conventional drug susceptibility test, by MGIT960, was used as reference. It also includes information pertinent to the sequence(s), including length and molecular weight. Please consider upgrading,
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This subsection of the 'Entry information' section provides one or more accession number(s). Streptomycin binds to the 30S ribosomal RNA subunit which results in decreased protein synthesis and misreading of mRNA.
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This subsection of the Names and taxonomy section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. Cyclic redundancy and other checksums
Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications.
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