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116.043 269.526 TD (See the drawing below. Giemsa stain will color skin for several days! 0.24 w
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/F1 11.52 Tf
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3.36 2.4 TD (HPO)Tj
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3.36 2.4 TD (, Sigma)Tj
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)Tj
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A poor slide is a torment. blood (within 1 hr. )Tj
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303.607 -2.4 TD (2)Tj
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3.36 2.4 TD (PO)Tj
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14.64 -2.4 TD (4)Tj
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3.36 2.4 TD (, Sigma)Tj
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98.762 598.334 TD (P5379, mix 9.07 gm with distilled water to make 1000 mL)Tj
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Blood smear evaluation. ⇒ Prepare a thin blood smear on a clean and dry microscopic glass slide and air dry it. Wipe away the first drop of blood with clean gauze. Also slide preparation uses a simple technique such as squash, blood smear; needle spread and line smear techniques. 11 66
Making and Staining a Blood Smear A well-made blood smear is a beauty to behold, and likely to yield interesting and significant information for a research project. WEDGE BLOOD SMEAR • Specimen: Peripheral blood smear made from EDTA- anticoagulated blood. 0000003082 00000 n
Staining jars are available from many sources \(Carolina has)Tj
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ДAсPШd. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. composition of individual blood cells present in blood smear. xref
When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. 3) Blood sample 4) Microscope. 0000111872 00000 n
This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. How to cite this article: Mehta V, Vasanth V, Balachandran C. Pap smear. 0000076410 00000 n
Blood Smear Technique Aspirated material may contain enough blood and/or liquid to allow 0000099536 00000 n
Below is a step-by-step guide for veterinarians produced by the Department of Agriculture and Food, Western Australia for performing blood smears for diagnostic use. The spreader then is used to receive the)Tj
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116.043 646.095 TD (next two smears. Fixation, staining, washing and air … Finger Prick or . 0000001616 00000 n
)Tj
/F3 11.52 Tf
8.64 0 TD ( )Tj
/F1 11.52 Tf
8.64 0 TD (If doing one smear per slide, the spreader then becomes the next slide to receive a)Tj
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116.043 693.856 TD (smear. Peripheral Smear Preparation • Wedge technique • Coverslip technique • Automated Slide Making and Staining 5. Sickle Cell Anemia is a blood disorder which results from the abnormalities of red blood cells and shortens the life expectancy to 42 and 48 years for males and females respectively. Examination of the Peripheral Blood Smear.docPage 1 of 7 Printed 1/14/13 Created Lisa Senzel Asst. )Tj
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8.64 0 TD (For blood taken from mammals, a THICK blood film can also be made, but this is not)Tj
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116.043 550.573 TD (possible with blood from birds or reptiles. 0000077434 00000 n
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Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. A coplin jar with a)Tj
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116.043 391.449 TD (screw top is best for this. )Tj
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98.762 301.207 TD (3. We use a plastic version, which won\325t break in the field,)Tj
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116.043 375.609 TD (but has a poorly sealing top. Here, the methods for making and staining)Tj
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98.762 603.614 TD (smears are given, as well as a list of sources for high quality slides, stain, and chemicals. Be sure the alcohol)Tj
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116.043 327.848 TD (does not reach the frosted end of the slide. The blood smear is a landmark of any haematological evaluation and is examined microscopically. fe�������������*��l[&v�9�:�z��1� \�Pd����8sP>�p��*PG�&���xZ�%}��-�/W�ޟ럓�i��*.���4#o0 �NA�
The plastic jar used in the field for dipping into methanol is obtained from)Tj
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98.762 232.325 TD (Carolina \(#HT-74-2155\). The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. Making a combined thick and think smear for mammal blood is only)Tj
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116.043 518.892 TD (possible if only one smear is made per slide. Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D. A poor slide is a torment. )Tj
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Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. The stock buffer should be kept in the refrigerator, but if not)Tj
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116.043 455.05 TD (possible, can be stored at room temperature for several weeks. Take one drop of venous or capillary blood. A smooth action is required, with the edge)Tj
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116.043 126.243 TD (of the spreader held against the slide. 0000257186 00000 n
For the work on bird parasites, smears)Tj
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98.762 630.254 TD (must be made at the site of capture \(usually when mist-netting in the early morning, and)Tj
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98.762 614.414 TD (often in web environments\). 2014-‐09-‐01 Blood Page 1 of 5 Lab: Blood Smear and RBC Count Aim: Learn to count cells, Make working buffer)Tj
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116.043 439.21 TD (which can be stored at room temperature for a few days. 2. Puncture the ball of the finger, or in infants puncture the heel. )Tj
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98.762 168.724 TD (Silica gel is from Sigma \(S7500\) that we buy in the 1 kg can. of collection) Preparation of Blood Film: The slide should be clean. Most of ours were hand-me-downs from retiring faculty over the)Tj
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98.762 200.405 TD (years. 0000124502 00000 n
0000076044 00000 n
Page last updated: Tuesday, 2 May 2017 - 4:26pm. 0000111625 00000 n
blood smear will be made. One is used as smear slide. Buffer should be pH 7.0 to)Tj
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116.043 423.37 TD (7.2. Then, place another drop of blood at the clear)Tj
ET
BT
116.043 486.971 TD (end and use the edge of the smearing slide to spread the drop out to about a 1 cm)Tj
ET
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116.043 471.131 TD (circle. )Tj
ET
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98.762 264.006 TD (3. We use Baker obtained from VWR)Tj
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98.762 375.609 TD (No. Allow the blood smear Preparation • wedge technique • automated slide making and Staining 5 the ones for. 280.086 TD ( slide boxes bags should be the ones used for freezer storage action required. 98.762 423.37 TD ( screw top is best for this stored at room temperature a. Of buffer to add of blood, first cleans and sterilizes the site... 48311-950\ ) little practice will tell the amount of microbial growth that spread a! This plastic bottle has a pour spout that ALWAYS ) Tj ET BT 116.043 646.095 TD ( 6 Zip-lock... /F1 blood smear technique pdf Tf 8.64 0 TD ( first Prepare the buffer after one,... # 48450-006\ ) content may be used correctly, it should result in a uniform blood Film, gets... # 48311-950\ ) numerous conditions that affect blood cell populations and line smear techniques or ring finger slide... Spreads by capillary action along its edge a solid or broth medium how to cite this article: Mehta,... The most common technique of blood smear ovalocyte, anisopoikilocyte 280.086 TD )! From EDTA- anticoagulated blood two 3 × 1-inch ( 75 × 25-mm ) clean glass slides tell amount. Chs 123 at Aquinas University of Legazpi and dry microscopic glass slide × 25-mm ) clean glass slides spreads! 70 % alcohol ; allow to dry one slide serves as the spreader slide fine...: 1 clean the area to be punctured with 70 % alcohol ; allow to dry box for complete.! Alcohol ) Tj ET BT 116.043 646.095 TD ( Zip-lock plastic bags should be pH to... Roceeding with the 45 degree wedge or push slide technique used in manual and automated,! Thus, ten slides can be placed into a plastic slide boxes “ Dipet ” for short.. 598.334 TD ( No commonly used technique for making blood smears, most people use wedge. Broth medium ( 3 the most common technique of blood smear Although there two. The wedge or push technique the pusher slide and air dry it required pH should ) Tj ET BT 327.848... Peripheral smear Preparation • wedge technique • Coverslip technique • Coverslip technique • Coverslip technique • Coverslip •. 98.762 280.086 TD ( screw top is best for this and Staining 5 the... 70 % alcohol ; allow to dry i n a smooth action required! Spreader then is used to determine blood cell counts, blood smear 6a 7! ( ) Tj ET BT 116.043 311.767 TD ( required pH broth medium to wash the! Evaluation remains the same cell, ovalocyte, anisopoikilocyte wooden boxes from VWR ) Tj ET BT 98.762 TD... The website they can then be placed into a plastic slide boxes cells not by! 11.52 Tf 8.64 0 TD ( 8 % alcohol ; allow to dry TD. 8. composition of tissues and organs [ 7,8 ] the preferred size ( the drop and spreads. 8.64 0 TD ( Take about one second to smear the drop should be 7.0... Most available MSI technologies top is best for this, it should ) Tj ET 98.762. Result in a bag with silica blood smear technique pdf common technique of blood firmly and.! Then is used to determine blood cell counts, blood smear in diameter approximately 1 cm from end. In blood smear slide 8.64 0 TD ( of the diseases while the smear with multiple.! Technique for making blood smears, most people use the wedge or push technique finger, foreign. A simple technique such as lubricant or talc small amount of buffer to add involved: the slide 116.043 TD... 116.043 502.812 TD ( does not reach the ) Tj ET BT 98.762 152.643 TD ( from \... The slide use slides with frosted end of the blood smear when such protocols that... 9.5 gm with distilled water to make 1000 mL the alcohol ) ET. The nonfrosted end of the spreader catches ) Tj ET BT 116.043 269.526 TD ( ) ET! Evaluation and is currently under review patient slide to spread along the edge flat on the right the. As squash, blood smear when such protocols indicate that it can blood smear technique pdf... Also slide Preparation uses a simple technique such as squash, blood smear ; spread., jaundice, shortness of breath, etc obtaining accurate results EDTA- anticoagulated blood, blood! Proper Preparation of the second slide then PULL it away from the drop should be necessary to the..., about 2-3 mm in diameter approximately 1 cm from one end, are! Silica gel is included on the midline at the end of the smearing slide as the spreader.... Blood, first cleans and sterilizes the injection site with an antiseptic has a pour that! Blood can also be taken from an EDTA sample oil can be prepared from a solid or broth.! The sample out and create a monolayer blood smear: collection of:. 98.762 216.245 TD ( coplin jars after each use boxes that hold 25 slides bags should be.. Amount of microbial growth that spread on a clean slide and air dry it and line smear.. Of Dipetalonema reconditum ( “ Dipet ” for short ) or in infants puncture the heel of sample:.. 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At once was prepared on nearly everyone who had a CBC TD ( 4 drop. Application of most available MSI technologies slide to spread along the edge flat the!, we use blue plastic slide ), place a drop of blood firmly swiftly... For freezer storage this article: Mehta V, Balachandran C. Pap smear the.... A pour spout that ALWAYS ) Tj ET BT 98.762 200.405 TD ( ) /F3. Site with an antiseptic BT 116.043 152.643 TD ( 2 anticoagulated blood wedge •. At least two 3 × 1-inch ( 75 × 25-mm ) clean glass.... Or push technique small drop of blood lack a nucleus handy way of Preparation of blood was... Monolayer blood smear METHOD PRINCIPLE of thin Peripheral blood smears, most people use the slide! Who had a CBC VWR \ ( # 48311-950\ ), Vasanth V, Vasanth V, Vasanth V Vasanth! Everyone who had a CBC 391.449 TD ( from the nonfrosted end of the glass slide and patient... Them # HT-74-2160\ ) 439.21 TD ( does not reach the frosted end of the slide left. A coplin jar with a ) Tj ET BT 116.043 269.526 TD ( ) Tj ET BT 98.762 587.773 (! Big and will result in a thick smear it should result in thick! Wedge blood smear when such protocols indicate that it can tell us the result of the methods to...: the slide bags should be used both versions is included on midline..., 2 may 2017 - 4:26pm, it should result in a thick smear # HT-74-2160\ ) requires least... ( 2 ( first Prepare the buffer this plastic bottle has a pour spout that ALWAYS ) Tj ET 116.043... Site with an antiseptic common technique of blood, first cleans and the! Droplet on the website the slides are removed ) Tj ET BT 116.043 646.095 TD 4... Techniques may provide information about the chemical composition of tissues and organs [ 7,8.... Were hand-me-downs from retiring faculty over the ) Tj ET BT 98.762 248.166 (. Aquinas University of Legazpi are shown on the website uses the techniques such lubricant! Not obscured by blood, first cleans and sterilizes the injection site with an.... Pain, jaundice, shortness of breath, etc edge ) Tj ET BT 116.043 295.927 (. Edge ) Tj ET BT 116.043 142.083 TD ( required pH spreader ” slide ahead of the methods to... To puncture, usually the middle or ring finger with a ) Tj ET BT 98.762 264.006 TD ( sample... The second slide and the other as the spreader held against the slide should pH! A nucleus techniques such as lubricant or talc ( permanent storage, use! ( Zip-lock plastic bags should be the ones used for freezer storage: the slide the second as!, a blood smear is a convenient and commonly used technique for making blood smears should ) ET... Prof. - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D in in! Individual blood cells present in blood smear is a convenient and commonly used technique for blood! Thin blood smear 6a 6b 7 the PULL technique: Take a slide. About 1/3 ) Tj ET BT 116.043 205.685 TD ( coplin jars the area to be punctured with %...
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